Page 68 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

P28

Effect of lipid raft disruption on AQP3, AQP5, and EFGR pathway

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Monika Mlinarić , Lidija Milković , Ana Čipak Gašparović
1 Ruđer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia

Background: Breast cancer is the leading cause of morbidity and mortality of cancer in women. Therefore,
there is a need for understanding modifications in biological processes and signaling pathways that
contribute to breast cancer malignancy. Oxidative stress is certainly an important factor that encourages
tumor growth by supporting several hallmarks of cancer. Peroxiporins are aquaporins that channel
hydrogen peroxide and could, therefore, play a role in regulating intracellular levels of oxidative stress,
consequently regulating important signaling pathways. Materials and methods: In this study, the effect of
lipid raft disruption on AQP3, AQP5, and EFGR pathway was explored. Lipid rafts were disrupted by
cholesterol depletion using methyl-β- cyclodextin. The effect of depletion was measured by MTT and BrdU
incorporation assay, and the effects on EGFR signaling pathway was assayed by Western blot levels of
pathway components. Results: Our results indicate that EGFR signaling pathway is affected by cholesterol
depletion. Conclusions: We have managed to disrupt lipid rafts to interfere with EGFR signaling and are
currently studying the consequences on signaling pathway components and levels of aquaporin 3 and
aquaporin 5.
Keywords: aquaporin, breast cancer, EGFR, lipid raft disruption.

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