Page 157 - SRPSKO DRUŠTVO ISTRAŽIVAČA RAKA

SDIRSACR Oncology Insights

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Cytotoxic activity of Thymus lykae Degen & Jav. (Lamiaceae) essential oil on human cancer cells in vitro

Marija Marin , Željko Žižak , Snežana Branković , Radmila Glišić 3
1
2
3
1Universty of Belgrade, Faculty of Biology, Belgrade, Serbia
2Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia
3University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, Serbia

Keywords: cancer cell line, cytotoxicity, essential oil, T. lykae

Background: T. lykae Degen & Jav. is an endemic species from the central Balkans, growing on dry, rocky serpentine
hills. Recent research has shown that the essential oil of wild T. lykae possesses antioxidant and antimicrobial activities
and could be used as a potential source of natural antioxidants in the food industry. The aim of this study was to
determine the cytotoxic potential of the essential oil of T. lykae on certain human cells in vitro: cervical cancer – HeLa,
the colon cancer – LS 174, the prostate cancer – PC-3 and the normal fibroblast cells – MRC-5.
Material and Methods: The essential oil was isolated from fresh plant material collected during the flowering period, in
Serbia. The air-dried plant parts were cut into small pieces and hydrodistilled for 2 hours using the Clevenger apparatus
to extract the essential oil. Cytotoxic activity was determined using the MTT colorimetric assay. Cells were seeded into
96-well microtiter plates and five different concentrations of oil diluted in RPMI-1640 medium were added to the wells.
Final dilutions applied to target cells ranged from 250 to 4000 x. The cultures were incubated for 72 h at 37 °C and then
20 μL of MTT solution (5 mg/mL in PBS) was added to wells. Number of viable cells in each well was proportional to the
intensity of formazan formed from the conversion of the MTT dye by viable cells.
Results: The results of the cytotoxic activity of the essential oil of T. lykae against cancer and normal cells were
determined as IC50 (half maximum inhibitory concentration – as average of dilution factors from three independent
experiments). IC50 [dilution] HeLa LS-174 PC-3 MRC-5 1:1998 1:2042 1:2032 1:2155 In all cancer cell lines, the essential
oil of T. lykae showed a strong anticancer effect, but the results showed no significant differences between the cancer
and normal cell lines.
Conclusions: This study is the first report on the potential antitumor activity of the essential oil of endemic wild T. lykae
on cancer cell lines and offers the possibility for further investigations on other cancer cell lines as well.

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Study of the anticancer activity of semisynthetic derivatives of sclareol

Εvangelia Papadogianni , Marialena Makrygianni , Igor Opsenica , Milica Pešić , Konstantinos Lafazanis , Costas
1
2
1
3
1
Demetzos , Εvangelos Manolopoulos , George Kolios , Κonstantinos Dimas 1
4
5
5
1Dep of Pharmacology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece
2Dep of Organic Chemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia
3Institute for Biological Research “Sinisa Stankovic” – National Institute of the Republic of Serbia, University of Belgrade, Belgrade,
Serbia
4Section of Pharmaceutical Technology, Dep of Pharmacy, NKUOA, Athens, Greece
5Lab of Pharmacology, Medical School, Democritus University of Thrace, Alexandropolis, Greece
Keywords: in vitro anticancer activity, slcareol, semisynthetic derivatives
Background: Sclareol, a natural substance found in many plants, exhibits significant anticancer properties, promoting
apoptosis and inhibiting cell proliferation. Purpose: We aimed to study the anticancer activity of semi-synthetic
derivatives of sclareol with 1,2,4-triazolo[1,5-a] pyrimidines.
Materials and Methods: 20 derivatives, synthesized and provided by the University of Belgrade (Faculty of Chemistry
and Institute for Biological Research “Siniša Stanković” – National Institute of the Republic of Serbia) were tested for
their in vitro anticancer activity. The SRB cytotoxicity method was used to study the effect of these substances on
the proliferation of four different human cancer cell lines: SF-268 (glioblastoma), NCI-H460 (large cell lung cancer),
MCF7 (breast cancer) and HCT-116 (colorectal carcinoma).The substances TNT396 and TNT397, as well as their four

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