Page 88 - SDIR5 Abstract book 21 12 2021.
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POSTER PRESENTATIONS
P48
Bee venom and melittin induce apoptosis in colon cancer cell lines by Caspase 8 activation
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Danijela Nikodijević , Jovana Jovankić , Danijela Cvetković , Aleksandra Nikezić , Stefan Blagojević ,
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Nevena Planojević , Milena Milutinović
1 University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Radoja Domanovića 12, 34000
Kragujevac, Serbia
2 University of Kragujevac, Institute for Information Technologies Kragujevac, Department of Natural Sciences,
Jovana Cvijića bb, 34000 Kragujevac, Serbia
Background: Animal venoms and their constituents shows ability to induce apoptosis, like a favorable way
of tumor cells elimination due to anticancer therapy. Aim of this study is to evaluate mechanism of
proapoptotic action of bee venom and melittin by determining Caspase 8 gene expression, as well as its
activity in colon cancer cell lines (HCT-116, SW-480, and HT-29). Material and Methods: Caspase 8 activity
was determined by colorimetric method (Caspase 8 colorimetric kit, RD Systems). Gene expression of
Caspase 8 was monitored by RT qPCR. Results: The results show that both treatments cause a significant
increase in the activity of Caspase 8 in colon cancer cell lines HCT-116, SW-480, and HT-29 (compared to
untreated cells). The gene expression of Caspase 8 under the influence of bee venom was increased in all
tested cell lines, while melittin causes its significant increase only in SW-480 and HT-29 cells. Conclusion:
Based on all results, activation of Caspase 8 indicates that in SW-480 and HT-29 cells apoptosis was initiated
by external death receptor-mediated pathway. Increasing of Caspase 8 gene expression in HCT-116 cells
was less pronounced compared to the other two. This correlates with results of other authors which
confirms that these cells require additional signals from the mitochondria for apoptosis activation.
Keywords: cancer, cell lines, colon, drug, venom, Caspase 8
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