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POSTER PRESENTATIONS



               P48



                 Bee venom and melittin induce apoptosis in colon cancer cell lines by Caspase 8 activation

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                  Danijela Nikodijević , Jovana Jovankić , Danijela Cvetković , Aleksandra Nikezić , Stefan Blagojević ,
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                                            Nevena Planojević , Milena Milutinović
                 1 University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Radoja Domanovića 12, 34000
                                                        Kragujevac, Serbia
                  2 University of Kragujevac, Institute for Information Technologies Kragujevac, Department of Natural Sciences,
                                             Jovana Cvijića bb, 34000 Kragujevac, Serbia

               Background: Animal venoms and their constituents shows ability to induce apoptosis, like a favorable way
               of  tumor  cells  elimination  due  to  anticancer  therapy.  Aim  of  this  study  is  to  evaluate  mechanism  of
               proapoptotic action of bee venom and melittin by determining Caspase 8 gene expression, as well as its
               activity in colon cancer cell lines (HCT-116, SW-480, and HT-29). Material and Methods: Caspase 8 activity
               was  determined by  colorimetric  method (Caspase 8  colorimetric  kit,  RD Systems).  Gene  expression of
               Caspase 8 was monitored by RT qPCR. Results: The results show that both treatments cause a significant
               increase in the activity of Caspase 8 in colon cancer cell lines HCT-116, SW-480, and HT-29 (compared to
               untreated cells). The gene expression of Caspase 8 under the influence of bee venom was increased in all
               tested cell lines, while melittin causes its significant increase only in SW-480 and HT-29 cells. Conclusion:
               Based on all results, activation of Caspase 8 indicates that in SW-480 and HT-29 cells apoptosis was initiated
               by external death receptor-mediated pathway. Increasing of Caspase 8 gene expression in HCT-116 cells
               was  less  pronounced  compared  to  the  other  two. This  correlates  with results of  other  authors  which
               confirms that these cells require additional signals from the mitochondria for apoptosis activation.
               Keywords: cancer, cell lines, colon, drug, venom, Caspase 8























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