Page 96 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

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Effects of vegf on molecular profile and invasiveness of human prostate cancer cells in vitro

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Taras Zadvornyi , Yelyzaveta Lukianova , Oleksandra Lykhova , Oleksandr Mushii
1 RE Kavetsky Institute of Experimental Pathology, Oncology, and Radiobiology, NAS of Ukraine; Kyiv, Ukraine.

Background: Prostate cancer (PCa) is one of the most common malignant neoplasms among men
worldwide. The main problem arising from PCa is its propensity to metastasize, in particular to the bones.
At the same time, the mechanisms of metastasis development associated with the influence of exogenous
factors, such as growth factors, remain unknown. We aimed to investigate the effects of exogenous VEGF
on the invasiveness and molecular profile of human prostate cancer cells with varying degrees of
malignancy. Material and methods: Human PCa cell lines (LNCaP and DU-145) were cultured with VEGF
(recombinant human VEGF-A, Abcam, UK). The invasive activity of the cells was examined using a standard
invasive test according to the manufacturer’s instructions. The expression levels of osteopontin,
osteonectin, Е-cadherin, N- cadherin, matrix metalloproteinase 2 (MMP-2), and MMP-9 were monitored
by immunocytochemical analysis. Results: We established that cultivation of human PCa cell lines with
VEGF increased invasive activity - by 42,5% (р<0,05) and 34,5% (р<0,05) in LNCaP and DU-145 cell lines,
accordingly. We found that under the action of VEGF there was an increase in the level of expression of N-
cadherin and matrix metallopeptidases (MMP-2 and -9). Furthermore, we observed the decrease of
expression of osteopontin in LNCaP cell lines and osteonectin in DU-145 cell lines. Conclusion: These results
suggest that exogenous VEGF stimulates the increase of the metastatic potential of PCa cells regardless of
their malignancy degree.
Key words: Prostate cancer, VEGF, invasive activity, osteopontin, osteonectin

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