Page 94 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

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The effects of Salinomycin on melanoma cell lines of different invasiveness

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Dušica Ristić , Milan Marković , Danijela Maksimović-Ivanić , Sanja Mijatović 1
1 Department of immunology, Institute for Biological Research "Siniša Stanković", National Institute of Republic of
Serbia, University of Belgrade

Background: Salinomycin belongs to the group of natural polyether antibiotics known as ionophores,
isolated from the bacteria Streptomyces albus, and exhibits a broad range of antibacterial, antifungal, and
antiviral activity. Furthermore, its anti-tumoral effects were investigated on a few cancer cell lines, and it
has been shown that Salinomycin inhibits division and metastatic potential and induce tumor cell death.
On the other hand, its effects on melanoma cell lines have not been investigated yet. Base on that, our goal
was to investigate the effect of Salinomycin on melanoma cell lines of the different stages of differentiation
and accordingly, level of aggressiveness. Material and methods: To find the correlation between
Salinomycin activity and aggressiveness of melanoma phenotype, viability, proliferation rate, and ROS
production were measured in highly invasive anaplastic A375 cells of human origin and two clones of B16
mouse melanoma cells, first F1 isolated from a solid tumor, and the second F10 isolated from lung
metastases. Having in mind that one of the most important environmental factors for tumor progression is
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the lack of oxygen, all tests were performed comparably in normoxic (37 C, 5%CO2, 20%O2) and hypoxic
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(37 C, 5%CO2, 6%O2) conditions in humidified chambers. Results: The results have shown that Salinomycin
strongly downregulated cell viability and inhibited proliferation of all melanoma cell lines. Its efficacy was
more profound in highly aggressive phenotype under both, hypoxic and normoxic conditions. Moreover,
its overall potential was amplified in a hypoxic environment for all tested cell lines. Both effects were tightly
related to the level of ROS production. Conclusion: Considering that Salinomycin potential was enhanced
in metastatic cell lines under hypoxia, this naturally occurring agent is a promising tool for the treatment
of advanced melanomas. Baring that in mind, further experiments should be done to prove the hypothesis
and light up this mechanism in detail.
Keywords: Salinomycin, melanoma, cell lines, culture, normoxia, hypoxia
Acknowledgements: Ministry of Education, Science and Technological development of the Republic of
Serbia No. 451-03-9/2021-14/200007.

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