Page 74 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

P34

Impact of TGFB1 Leu10Pro polymorphism on acute radiotherapy-induced toxicity in prostate
cancer patients

1,2
Emina Mališić , Nina Petrović , Marina Nikitović 3,4
1
1 Institute for Oncology and Radiology of Serbia, Department of Experimental Oncology, Belgrade, Serbia
2 “VINČA“ Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, Belgrade,
Serbia
3 Institute for Oncology and Radiology of Serbia, Department of Radiation Oncology, Belgrade, Serbia
4 Faculty of Medicine, University of Belgrade, Belgrade, Serbia

Background: Radiotherapy (RT)-induced acute toxicity associated with bladder and bowel injury has great
impact the quality of life for prostate cancer (PCa) patients. TGFB1 is a key proinflammatory and profibrotic
cytokine, but its role in acute toxicity is still unclear. TGFB1 T>C transition at codon 10 results in leucine to
proline substitution and increased TGFB1 protein levels. The aim of this study was to examine impact of
TGFB1 Leu10Pro (rs1800470) polymorphism on RT-induced acute toxicity in PCa patients. Patients and
methods: Eighty two patients who had a localized or locally advanced PCa were treated with radical (72
Gy)(n=47) or postoperative/salvage (66 Gy)(n=35) RT without previous hormonal therapy. TGFB1 Leu10Pro
was determined by PCR-RFLP analysis on DNA from PBMC. The differences in the distribution of genotypes
for dominant, recessive, codominant and overdominant genetic model between patients with or without
acute genitourinary (GU) or gastrointestinal (GI) toxicity as well as different grade of toxicity were tested
by χ2 and Fisher’s exact test. P values ≤0.05 were considered statistically significant. Results: Heterozygote
PCa patients had lower rate of acute GU and GI toxicity then homozygotes (LeuLeu, LeuPro, ProPro were:
100%, 90.7%, 100% for GU and 92.0%, 88.4%, 100%, respectively for GI). The frequency of toxicity grade
≥2 were higher in LeuPro then both homozygote carriers (41% vs. 28.2% for GU and 26.3% vs. 21.6% for GI
acute toxicity). The differences were not statistically significant. Conclusion: The present study did not
establish impact of TGFB1 Leu10Pro polymorphism on RT-induced acute toxicity in PCa patients.
Keywords: polymorphism, prostate cancer, radiotherapy, toxicity

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