Page 78 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

P38

Clinopodium nepeta (L.) Knutze essential oil in-vitro anti-proliferative activity on PC-3, Du145
and LS174 human cancer cell lines

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1
Emina Boškailo , Hurija Džudžević-Čančar *, Tatjana Stanojković , Igor Jerković , Željko Žižak , Alema
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Dedić , Anesa Jerković-Mujkić , Alispahić Amra
1 Departnent of Pharmacy, Faculty of health studies, University of modern sciences-CKM, Mostar, Bosnia and
Herzegovina
2 Department of Chemistry in Pharmacy, Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and
Herzegovina
3 Institute of Oncology and Radiology of Serbia, Belgrade, Serbia
4 Division of Chemistry, Faculty of Chemistry and Technology, University of Split, Split, Croatia
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Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Background: Due to the diversity of chemical structures of natural products, it has been observed that some
components often individually or in synergy with others have a toxic effect on malignant cells. Plants of the
genus Clinopodium are known in folk medicine for their contribution to human health. Material and
methods: Essential oils of wild Clinopodium nepeta (L.) Knutze from southern Bosnia and Herzegovina, from
various different habitats (Pirići, Lokve, Blagaj and Počitelj), were the subject of this research. Using a
modified Clevenger apparatus essential oils (EOs) samples were obtained by hydrodistillation. Qualitative
and quantitative analysis was performed by GC-MS. EOS effect on PC-3, Du145, and LS174 cancer cell
survival was determined calorimetrically using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium
bromide dye (MTT assay). Human cancer cell lines used were obtained from the American Type Culture
Collection (Mansas, VA, USA). Results: Chemical characterization revealed trans-piperitenone oxide in
samples from Lokve and Počitelj (60,03 and 51,66%) and pulegone in samples from Pirići and Blagaj (23,17
and 43,86%) as predominant compounds. The results of cells proliferation measurments by MTT assay,
expressed as IC50 (EOs concentration in µg/mL inhibiting cell survival by 50%), indicated high toxicity to
cancer cells. The EOs of C. nepeta showed strong antiproliferative activity (IC50=1.55±0.27 to 17.42±0.81
µg/mL) against all three cell lines. In general, all four tested EOs samples showed the best activity against
LS174 cells, especially the samples from Lokve and Počitelj (1.71±0.58 and 1.55±0.27 µg/mL). Conclusions:
Presented dates can be interpreted by the existence of a positive correlation between the presence of
certain components in EOs and their antiproliferative activity. To the best of our knowledge, no other work
has produced results that show the effects of clinopodium nepeta EOS on the mentioned human cancer
cell lines. Therefore, this research can serve as a good preliminary guideline for planning of in vivo studies
on the same and other cancer cells.

Keywords: Lamiaceae, Clinopodium nepeta (L.) Knutze, essential oil, chemical composition, anti-
proliferative activity, MTT assay.

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