Page 76 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

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NF-κB as common target gene of miRNAs related to oxidative stress and prostate cancer
radiotherapy response

1,2
2
Nina Petrović and Ana Đurić
1 Laboratory for Radiobiology and Molecular Genetics, Department of Health and Environment, “VINČA“ Institute of
Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, Mike Petrovića Alasa 12-14,
11351 Belgrade, Serbia
2 Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Pasterova 14, 11000
Belgrade, Serbia

Background: Between 50% and 60% of cancer patients receive radiotherapy (RT). Oxidative stress (OS),
DNA repair, and hypoxia in combination with individual genetic/epigenetic background are major events
influencing the individual response to radiation exposure. Deleterious effects of irradiation (IR) are strongly
associated with OS. Oxidative stress occurs when cells fail to maintain balance between the production and
scavenging of reactive oxygen species (ROS). Overproduction of ROS is associated with side effects of RT.
MicroRNAs are small non-codding RNA involved in a wide variety of cellular processes and radiation
response amongst others. Our aim was to investigate common target gene silenced by the highest number
of miRNAs involved in oxidative stress during RT. Material and methods: Two approaches have been used
to elucidate miRNA network and common target genes-literature survey and bioinformatics analysis with
miRNet online software. Based on literature survey results, we performed multiple combinatorial queries
for hsa-miR-17-3p/21/34a/96/122/146a/155/193a/200a/206/210 involved in oxidative stress in response
to IR/RT in PCa. Results: Common target of the highest number of miRNAs (4)-miR-21/34a/146a/155 was
nuclear factor-κB (NF-kB). According to “Function Explorer search” of miRNet, KEGG pathway listed
molecules belonging to signaling pathway involved in PCa apoptosis regulation. Conclusion: The most
promising miRNAs which should be investigated further in response to RT and oxidative stress in PCa are
miR-21/34a/146a/155, and especially its interaction with NF-kB. Transcription factor NF-κB is considered
as crucial link between inflammation and cancer and can be activated by ROS and IR. Modulating NF-κB
signaling in PCa cells may influence on response to RT.
Keywords: MicroRNA; Oxidative stress; Prostate cancer; Radiotherapy

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