Page 82 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

P42

Antineuroblastoma potential of polyoxopalladate(II)

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1
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Andjelka M. Isakovic , Marija Jeremic , Danijela Krstić , Mirjana B. Čolović , Ulrich Kortz , Sonja Misirlic-
Dencic 1
1 Institute of Medical and Clinical Biochemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
2 Institute of Medical Chemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
3 Department of Physical Chemistry, “Vinča” Institute of Nuclear Sciences-National Institute of the Republic of
Serbia, University of Belgrade, Belgrade, Serbia
4 Department of Life Sciences and Chemistry, Jacobs University, Bremen, Germany

Background: Polyoxometalates are a class of anionic, polynuclear metal-oxo clusters reported as promising
in vitro and in vivo antitumor agents for several decades. The aim of this study was to investigate the
antineuroblastoma potential of the polyoxopalladate(II) nanocube Na8[Pd13As8O34(OH)6]・42H2O (Pd13).
Material and methods: All experiments were performed on human neuroblastoma cell line, SH-SY5Y. The
number of viable cells after the treatment with Pd13 was assessed using an acid phosphatase viability assay.
The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, acidic intracellular
vesicles content, and the cell cycle was determined by flow cytometry. Results: The obtained results suggest
that Pd13 caused a significant decrease in cell viability with IC50 values of 7.7 µM (24 h) and 4.4 µM (48 h).
Pd13 induced depolarization of mitochondrial membrane (2 h), followed by ~ 30% increase in the production
of the superoxide ion (O2-) 4 h after treatment. An increase (~ 30%) in pancaspase activation and
disturbance of neuroblastma cell cycle were observed after 24 h treatment. Namely, Pd 13 caused an
increase (14.4%) in the number of cells with fragmented nuclear DNA (SubG0), a decrease (%) of cells in the
G1 phase, and an increase (%) in the S phase, all suggestive of cell cycle arrest. Finally, Pd 13 increased the
orange to green fluorescence ratio for ~ 45% 24 h after treatment, supporting intracellular acidification.
Conclusion: The polyoxopalladate, Pd13 can be regarded as a promising antineuroblastoma agent which
induces oxidative stress, and causes pan-caspase activation, DNA fragmentation and cell cycle arrest, which
are all hallmarks of apoptotic neuroblastoma cell death.
Keywords: polyoxopalladates, antitumor, neuroblastoma, apoptosis

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