Page 81 - SDIR5 Abstract book 21 12 2021.

POSTER PRESENTATIONS

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Screening of the cytotoxic, antibacterial and antifungal activities of Saccorhiza polyschides
algae extract

Nađa Grozdanić , Marijana Kosanić , Samira Etahiri , Omar Assobhei , Jamila Benba ,Tatjana P.
1
2
3
3
3
1
Stanojković
1 Institute for Oncology and Radiology of Serbia, Belgrade, Serbia,
2 University of Kragujevac, Faculty of Science, Department of Biology, Kragujevac, Serbia
3 Université Chouaib Doukkali, Faculté des Sciences, El Jadida, Morocco

Background: Marine macroalgae are well known for their anticancer and antimicrobial potentials as well as
a benefit to overall human health after consumption. They are rich in bioactive compounds such as
polysaccharides, fatty acids, pigments, terpenes and phenolic compounds. Material and Methods:
Saccorhiza polyschides was collected from the Atlantic coast of Morocco (Sidi Bouzid coast).
Dichloromethane-methanol extract of the algae was tested for in vitro cytotoxicity by MTT assay against
human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and
chronic myelogenous leukemia (K562) cell lines. Antibacterial and antifungal activities were analyzed using
micro-dilution method to determine minimal inhibitory concentration (MIC). Bacterial species used were:
Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli and Proteus mirabilis. Fungal
species used were: Mucor mucedo, Trichophyton mentagrophytes, Aspergilus niger, Candida albicans and
Penicillium italicum. Results: Extract showed high cytotoxic activity for natural products in vitro toward
malignant cell lines. The highest cytotoxic activity of S. polyschides extract was exerted toward K562
(48.45±0.72 µg/ml) and HeLa (67.87.45±4.42 µg/ml) cell lines. Antibacterial activity was most pronounced
against B. subtilis and B. cereus (3.45 mg/ml) bacteria. Antifungal activity of the extract was the highest
toward C. albicans (3.45 mg/ml) fungus. Conclusion: Results showed promising cytotoxic antibacterial and
antifungal activities of the S. polyschides extract, however further tests are needed to confirm these actions
and to determine active components that are responsible for these actions.
Keywords: algae, cytotoxic, drug, antimicrobial, in vitro, anticancer

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